Not known Factual Statements About thr77
Not known Factual Statements About thr77
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The antibody is from purified rabbit serum by affinity purification through sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
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Overexpression of the gene has become observed in many tumors, which leads to chromosome instability, and therefore might contribute to tumorigenesis. This protein was uncovered to associate with, and be linked to, the phosphorylation of NPAT protein (nuclear protein mapped on the ATM locus), which participates in cell-cycle controlled histone gene expression and plays a critical purpose in selling cell-cycle progression from the absence of pRB. Two alternatively spliced transcript variants of the gene, which encode distinctive isoforms, are already described. Two supplemental splice variants have been reported but in-depth nucleotide sequence data is not still accessible.
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Blocking peptides are peptides that bind particularly on the goal antibody and block antibody binding. These peptide ordinarily includes the epitope regarded through the antibody.
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This guideline outlines even further achievable optimization of cellular and immunoassay parameters to be certain the very best final results are...
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This guideline outlines further possible optimization of cellular and immunoassay parameters to ensure the best click here possible effects are...
Antibodies certain to the blocking peptide now not bind on the epitope about the focus on protein. By comparing the staining from your blocked antibody versus the antibody alone, you can see which staining is unique.
Focus on Data Cyclin E belongs on the very conserved cyclin loved ones, whose members are characterised by a extraordinary periodicity in protein abundance throughout the cell cycle. Cyclins function as regulators of CDK kinases. Distinctive cyclins show unique expression and degradation designs which contribute into the temporal coordination of every mitotic occasion. Cyclin E forms a posh with and functions as a regulatory subunit of CDK2, whose action is needed for cell cycle G1/S changeover. This protein accumulates at the G1-S phase boundary and is degraded as cells development by way of S stage.
The measurement of protein phosphorylation is a useful tool for measuring the modulation of receptor activation by equally antibodies...
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